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| Arena,Gabriella Dias; Ramos-González,Pedro Luis; Nunes,Maria Andréia; Jesus,Camila Chabi; Calegario,Renata Faier; Kitajima,Elliot Watanabe; Novelli,Valdenice Moreira; Freitas-Astúa,Juliana. |
| ABSTRACT: Brevipalpus-transmitted viruses (BTV) are a taxonomically diverse group of plant viruses which severely affect a number of major crops. Members of the group can be sub-classified into cytoplasmic (BTV-C) or nuclear type (BTV-N) according to the accumulation sites of virions in the infected plant cells. Both types of BTV produce only local infections near the point of inoculation by viruliferous mites. Features of BTV-plant interactions such as the failure of systemic spread in their natural hosts are poorly understood. In this study we evaluated Arabidopsis thaliana, a model plant commonly used for the study of plant-virus interactions, as an alternative host for BTV. Infection of Arabidopsis with the BTV-N Coffee ringspot virus and Clerodendrum... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Clerodendrum chlorotic spot virus; Cilevirus; Solanum violaefolium ringspot virus; Coffee ringspot virus; Dichorhavirus. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162017000100085 |
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| Palmieri,Darío Abel; Novelli,Valdenice Moreira; Bastianel,Marinês; Cristofani-Yaly,Mariângela; Astúa-Monge,Gustavo; Carlos,Eduardo Fermino; Oliveira,Antonio Carlos de; Machado,Marcos Antonio. |
| Nearly 65,000 citrus EST (Expressed Sequence Tags) have been investigated using the CitEST project database. Microsatellites were investigated in the unigene sequences from Citrus spp. and Poncirus trifoliata. From these sequences, approximately 35% of the non-redundant ESTs contained SSRs. The frequencies of different SSR motifs were similar between Citrus spp and trifoliate orange. In general, mononucleotide repeats appeared to be the most abundant SSRs in the CitEST database, but we also identify di-, tri-, tetra-, penta- and hexanucleotide repeats. The AG/CT and AAG/CTT were the most common dinucleotide and trinucleotide motifs, with frequencies of 54.4% and 25.2%, respectively. Primer sequences flanking SSR motifs were successfully designed and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Citrus spp.; Poncirus trifoliata; ESTs; Microsatellites; In silico polymorphism. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500029 |
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| Novelli,Valdenice Moreira; Machado,Marcos Antonio; Lopes,Catalina Romero. |
| Isoenzymatic polymorphism analysis was used to determine genetic variability among species and hybrids of Citrus spp. and one accession of Poncirus trifoliata (L.) Raf. Ten enzymatic systems aspartate aminotransferase (AAT), acid phosphatase (ACP), leucine aminopeptidase (LAP), 6-phosphogluconate dehydrogenase (6-PGD), isocitrate dehydrogenase (IDH), phosphoglucoisomerase (PGI), phosphoglucomutase (PGM), diaphorase (DIA), shikimate dehydrogenase (SKD) and peroxidase (PRX) were analyzed. Twenty loci and 48 alleles were identified. Sweet orange cultivars (C. sinensis (L). Osbeck) showed the highest polymorphism with the largest number of heterozygous loci, although the alleles of those loci were the same in all cultivars, with the exception of Westin and... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100030 |
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| Freitas-Astúa,Juliana; Fadel,André Luiz; Bastianel,Marinês; Novelli,Valdenice Moreira; Antonioli-Luizon,Renata; Machado,Marcos Antônio. |
| O objetivo deste trabalho foi buscar novas fontes de resistência à leprose-dos-citros, no Banco Ativo de Germoplasma do Centro APTA Citros Sylvio Moreira, Instituto Agronômico, em Cordeirópolis, SP. Foram utilizadas plantas obtidas por sementes de 26 acessos, infectadas com o vírus da leprose-dos-citros (Citrus leprosis virus - CiLV), por meio do seu vetor Brevipalpus phoenicis. O aparecimento de lesões, a partir de 21 dias após a inoculação, foi observado em 11 dos genótipos testados (42,3%). Quinze espécies, entre elas Citrus pennivesiculata e C. celebica, comportaram-se como altamente resistentes, enquanto outras, como C. keraji, foram mais suscetíveis que o padrão C. sinensis. Os dados mostraram grande variação de respostas de Citrus spp. à leprose,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brevipalpus phoenicis; Citrus spp.; CiLV; Melhoramento genético; Resistência genética. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000700004 |
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